Green Biotec GmbH Germany

DrMuhammadFarrukhNisar

Dr Muhammad Farrukh Nisar

Principle Scientist

Dr. Muhammad Farrukh Nisar is a highly accomplished and dynamic Assistant Professor of Physiology and Biochemistry with over eight years of distinguished experience in academia and research. Currently serving at the Department of Physiology and Biochemistry, Cholistan University of Veterinary and Animal Sciences (CUVAS), Bahawalpur, Pakistan, he has also held significant leadership roles, including Head of Department and Director of the Office of Research, Innovation, and Commercialization (ORIC).

Dr. Nisar’s academic credentials are robust, featuring a Post Doctorate from Jiangxi Agricultural University, China, a Ph.D. in Biomedical Engineering from Chongqing University, China, and an M.Phil. in Cell and Molecular Biology from Quaid-i-Azam University, Islamabad. His research is highly influential, evidenced by an impressive h-index of 30 and over 2,669 citations (Google Scholar), reflecting his significant contributions to the scientific community.

His primary research interests lie at the cutting edge of biomedical science, focusing on:

  • Cancer Biology & Therapeutics: Investigating natural anti-cancer drugs, small drug molecules, and non-coding RNAs (lncRNAs, microRNAs) to induce cell death mechanisms like apoptosis, autophagy, and ferroptosis in tumor cells.

  • Computational Drug Design: Utilizing molecular docking, network pharmacology, and bioinformatics for novel drug discovery and development.

  • Oxidative Stress & Photomedicine: Exploring the Nrf2/HO-1 cytoprotective pathway and the therapeutic use of ultraviolet radiation (UVA) to combat oxidative stress-related diseases and photoaging.

  • Nanomedicine & Drug Delivery: Developing advanced biomedical materials and nanoparticle-based systems for targeted drug delivery and enhanced therapeutic efficacy.

A dedicated educator and mentor, Dr. Nisar has successfully supervised numerous M.S. students and is proficient in a wide array of advanced laboratory techniques, including Western Blotting, qRT-PCR, Flow Cytometry, Confocal Microscopy, and Cell Culture. His work is supported by competitive grants, such as an HEC Start-up Research Grant.

Dr. Nisar is an active participant in the global scientific dialogue, having been a guest speaker at international conferences and successfully completing an ERASMUS+ Teaching Mobility program at the University of Siedlce, Poland. His scholarly output is prolific, with over 75 publications in high-impact international journals, including Signal Transduction and Targeted Therapy, Oxidative Medicine and Cellular Longevity, and The FEBS Journal. He also serves as an editor for several prestigious books and a guest editor for special issues in renowned scientific journals.

Driven by a mission to accept challenges and work in a dynamic environment, Dr. Nisar is a fluent English speaker and a skilled scientific writer and project manager, committed to advancing human health through innovative research and education.

Contact: mfarrukhnisar@cuvas.edu.pk | farrukh.nisar@hotmail.com
ORCID: 0000-0001-8873-1227
Google Scholar: Dr. Nisar’s Profile

EDUCATIONAL BACKGROUND

  • Post Doctorate [06.05.2025], Jiangxi Agricultural University, Nanchang, 330035, Nanchang, China.
  • Ph.D. [23.12.2016] Biomedical Engineering, Chongqing University, Chongqing, 400044, P.R. China.
  • M.Phil. Cell and Molecular Biology, Quaid-i-Azam University, Islamabad, Pakistan.

AREAS OF INTEREST FOR TEACHING AND RESEARCH

  • Natural Anti-cancer drugs, small drug molecules, non-coding RNAs (lncRNAs, microRNAs, RNAi, etc.).
  • Molecular Docking and simulation, Network pharmacology, Computational biochemistry for drug designing, Nanomedicine.
  • Apoptosis, Autophagy, NETosis, Ferroptosis, Senescence, ER stress in tumor cells, tumor stem cells, etc.
  • Oncology/Cancer biology of the cell, tumorigenesis, and tissue engineering.
  • Biochemical Pathways (Cell signaling pathways), Oxidative stress and cellular defense mechanisms through Nrf2/HO-1, cytoprotective & cell death mechanisms.
  • Free Radicals Biology (ROS/RNS), and physiological role in different diseases.
  • Photomedicine, Photo- and Chemo-therapies to treat cancer cells, photoaging.
  • Biomedical Materials for targeted drug delivery, Phytochemistry & TCM.

EXPERIENCE

Total University teaching/Research experience over 8 years.

  • Assistant Professor (03.12.2018 - date) Department of Physiology and Biochemistry at Cholistan University of Veterinary and Animal Sciences (CUVAS), Bahawalpur, Pakistan.
  • Head of Department of Physiology and Biochemistry, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan. (02.01.2023 to 30.04.2024).
  • Director of the Office of Research, Innovation, and Commercialization (ORIC) from 01.01.2022 to 03.01.2023.
  • Assistant Professor at interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore, Pakistan. October, 2017 to December, 2018.
  • Research and Teaching Assistant in Biochemistry lab at College of Bio Engineering, Chongqing University, Chongqing (400044), China. (Three and Half years)
  • Lecturer at University of Gujrat, Pakistan [16.09.2009 - 28.02.2011] to post graduate classes (M.Sc. & BS Hons).
  • Research Associate in Cell and Molecular Biology Lab in QAU, Islamabad, Pakistan [2006-2008].

DISTINCTIONS

  1. Successfully completed the ERASMUS+ Teaching Mobility program at University of SIEDLCE, Poland (27.01.2025 – 31.01.2025).
  2. Awarded with 1st Position in the poster competition for presenting a poster entitled, "Natural Small De-Ironization Inducer (DII) Drug Molecules For Medicated Skin Care Products" at 2nd International Conference on Recent Innovations in Plant Sciences and Natural Products, 15 - 16th February, 2024, University of Okara, Okara, Pakistan.
  3. Awarded with 1st Position in the poster competition for presenting poster entitled, "The biomedical application of phytochemicals as de-ironization inducers (DII) in medicated skin care products" at First International Conference on General Chemistry 10th-11th February 2022, organized by Department of Chemistry, COMSATS University Islamabad, Islamabad campus, Islamabad, Pakistan.
  4. Awarded with 2nd Position in poster competition for presenting poster entitled, "Development of artificial skin for second degree burn wounds using chia seeds gel" in 2nd Two-Day National Training Workshop on "Bioassays for the exploration of Bioactives" during 25-26th October, 2021, conducted by Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
  5. Awarded with 1st Position in the poster competition for presenting porter entitled, "Development of novel biomedical material using Chia seed gel combined with plant extracts for antibacterial, wound healing and drug delivery. Presented in Poster Presentation Competition on One World One Health Day, organized by One Health Club, CUVAS, Bahawalpur, Pakistan. 03.11.2020.
  6. Awarded with 3rd Position in the poster competition for presenting poster entitled, Ultraviolet radiation A: Modulation of oxidative stress responsive Nrf2/HO1 pathway to combat CoVID-19. Poster presented in Poster Presentation Competition on One World One Health Day, organized by One Health Club, CUVAS, Bahawalpur, Pakistan. 03.11.2020.
  7. Awarded with Chinese Government Scholarship for the Ph.D. study during 09.2013 to 12.2016, at College of Bioengineering, Chongqing University, Chongqing, 400044, China. Availed an exchange program of University of Bath, UK, with Chongqing University, China.
  8. Silver Medal in M.Sc. university exam [2005], in Islamia University annual examination, 2005. Merit Certificate awarded by IUB at 2nd Convocation in 2009. Certificate No. 1089, issued on 10.03.2006.
  9. Awarded Best Student Certificate in Govt. Sadiq Egerton College at the eve of 125th Anniversary, 2010.
  10. A certificate of performance for securing 2nd position in the university exam, and SE College at the eve of 125th Anniversary 2010 of Govt. Sadiq Egerton College, Bahawalpur, 2010.
  11. Participated in 2nd National Youth Convention, held at Jinnah Convention Center Islamabad, 18-19 July 2005, from Bahawalpur district.

LANGUAGE PROFICIENCY

Fluent in all Four Modules of English (Native Level, British phonetics)

SKILLS

Techniques being used in Biochemistry (Cell Biology, Molecular Biology, and biorheology) labs:

Molecular & Cellular Techniques

  • Western Blotting (Immunoblotting)
  • qRT-PCR
  • Confocal Microscopy
  • semi qRT-PCR
  • cDNA PCR
  • Cell Culture techniques (2-D/3-D)
  • ELISA
  • Flow cytometry (Apoptosis, Cell cycle assay, Autophagy, ROS)
  • Colony Formation assay
  • Zymography (Gelatin and Casein)
  • Gel electrophoresis

Specialized Techniques

  • UVA/UVB/UV/CAP irradiations
  • ROS (Reactive Oxygen species) Detection Assays
  • Cell Damage Analysis assays (MDA/LDH/SOD)
  • Cell Viability/Cytotoxicity Assays
  • Transfection of target genes and Gene Silencing
  • Immunofluorescence assay
  • Cell migration assay
  • Xenograft assays in animal models
  • Treatment/handling of animal model

Computational & Analytical Skills

  • Molecular Docking and Network Pharmacology
  • Preparation of biomedical materials and characterization
  • Molecular docking and simulation
  • Network pharmacology tools
  • Data analytics using R
  • Meta-analysis
  • Drug designing and toxicological evaluations

Professional Skills

  • Expert in scientific writings
  • Project designing and management
  • Proficient in computer and basic applications
  • Excellent in verbal communication
  • Preparation of routine lab consumables/reagents/media etc.

RESEARCH PROJECTS

Completed: HEC- Start-up research grant program approved on 09.01.2018.

Title: The Protective Effects of Encapsulated Quercetin@ZnO Against UVA-induced Photodamage in Human Epidermal Keratinocytes (HEK) by Activation of eIF2α Phosphorylation.

Grant approved: 478,098.00 PKR. (Award Letter No. 21-1819/SRGP/R&D/HEC/2018).

STUDENTS SUPERVISED

Co-supervised one MS student under research visitor program of IRCBM, CUI Lahore Campus. The student worked on the preparation and characterization of the basic materials for the anti-aging skin creams; prepared various sized drug loaded ZnO nanoparticles. Further, conducted the cell studies for these drug-loaded ZnO NPs.

14 MS Students has already supervised From 2018-2024, and 10 are in progress.

PUBLICATIONS: Journal Articles

Sr. # Authors Name Title Name of Journal Volume No. /Page No. Date (M.Y) Impact Factor
1 M Dilawar, A Malik, J Liao, S Lin, P Zhang, J Wang, T Wu, N Zhang, Muhammad Farrukh Nisar*, G Chen* *co-corresponding Authors Decoding the IGF/IGFR axis: from cellular homeostasis to disease pathogenesis and therapeutic frontiers Signal Transduction and Targeted Therapy Under Minor Revision 19.08.2025 52.8
2 X Deng, T Hou, Muhammad Farrukh Nisar, X Zhang, Y Yang, C Chen, J Chen Investigating the Antifungal Mechanism of Hinokitiol as a novel antifungal agent: Understanding toxicity towards Geotrichum citri-aurantii and efficacy in controlling citrus sour rot Phytopathology Online doi: 10.1094/PHYTO-03-25-0119-R 25.06.2025 3.1
76 CC Wan, X Hu, P Yang, M Li, Muhammad Farrukh Nisar, Z Zhou, Y Gan, Y Cai, Z Liu Mechanism of theaflavins inhibiting proliferation of nasopharyngeal carcinoma cells Food Science and Nutrition In Revision 10.03.2025 3.5

Note: Only the first 2 and last publication are shown here. The full table includes 76 publications.