Bulk RNA-Seq Analysis
Description
Embark on a comprehensive journey into RNA sequencing (RNA-Seq) analysis with our intensive workshop tailored for researchers, bioinformaticians, and data scientists eager to master this powerful genomic technique. RNA-Seq has transformed our understanding of transcriptomics, offering unparalleled insights into gene expression patterns and regulatory mechanisms across diverse biological systems.
In this workshop, you’ll explore the core principles and advanced methodologies of RNA-Seq analysis, covering everything from raw data processing and quality assessment to differential expression analysis and functional interpretation. Through a combination of lectures, guided tutorials, and hands-on exercises, you’ll develop the skills to analyze and interpret RNA-Seq data using industry-standard tools such as DESeq2, edgeR, and GSEA.
Key Highlights:
Introduction to RNA-Seq
- Understand the principles and applications of RNA sequencing technology
- Compare RNA-Seq to other gene expression analysis methods
Raw Data Processing and Quality Control
- Assess the quality of raw sequencing data using FastQC
- Perform read trimming and filtering to improve data quality
Read Alignment and Quantification
- Align reads to a reference genome using popular tools like HISAT2 or STAR
- Quantify gene expression levels using tools such as featureCounts or Salmon
Data Normalization and Batch Effect Correction
- Apply various normalization methods (e.g., TPM, RPKM, DESeq2’s median of ratios)
- Identify and correct for batch effects using tools like ComBat or SVA
Exploratory Data Analysis
- Perform and interpret principal component analysis (PCA) on RNA-Seq data
- Create and analyze sample-to-sample correlation heatmaps
Differential Expression Analysis
- Conduct differential expression analysis using DESeq2 or edgeR
- Interpret volcano plots and MA plots to visualize differential expression results
Functional Enrichment Analysis
- Perform Gene Ontology (GO) and pathway enrichment analysis
- Visualize enrichment results using tools like Enrichr or g:Profiler
Advanced Analysis Techniques
- Explore gene co-expression networks
- Conduct gene set enrichment analysis (GSEA) for hypothesis testing
Who Should Attend: .
This workshop is perfect for molecular biologists, geneticists, bioinformaticians, and any researchers interested in transcriptome analysis and functional genomics.
Harness the full potential of RNA-Seq to unravel the complexities of gene expression. Join us for this in-depth workshop and elevate your genomic data analysis capabilities to new heights!
Organizer
Dr. Amir Hussain is a distinguished Senior Bioprocess/Biochemical Engineer with over a decade of experience in biotechnology, bioprocess engineering, and microbiology. His career is marked by significant contributions to both research and academia, with a strong focus on enzyme and cell cultivation, biotransformation, and fermentation. Dr. Hussain’s expertise extends to advanced training in upstream and downstream processes, where he has successfully guided undergraduate and graduate students through complex biotechnological applications. Dr. Hussain’s educational background includes a Ph.D. in Biochemical Engineering from Jacobs University Bremen and a Master’s degree in Agricultural Sciences from Faisalabad Agriculture University, Pakistan. His extensive skill set, combined with his practical experience in both academic and industrial settings, makes him a leader in the field of biotechnology and a sought-after trainer in bioprocess development.
Dr. Amir Hussain is a recognized innovator in the field of bioprocess engineering, with a proven track record of advancing the industry through his research and academic contributions. He has authored eight scientific publications and successfully managed a European Union project with a €250,000 lab grant. Dr. Hussain’s work demonstrates a deep understanding of current industry trends, coupled with a strong commitment to driving innovation in bioprocess development and biotechnology.
Currently based in Bremen, Germany, Dr. Hussain serves as a Senior Bioprocess Development Scientist at Green Biotec UG. In this role, he manages multifaceted projects that include genetic and cell line engineering, bioreactor design, and process optimization. His research has been integral to European Union-funded projects, where he developed innovative bioprocess solutions, particularly in the scale-up and transfer of microbial and mammalian cell cultivation. His work is widely recognized, with multiple scientific publications and a substantial grant under his belt.
Dr. Rehan Zafar Paracha is a renowned interdisciplinary scientist and academic, currently serving as a Tenured Associate Professor at the School of Interdisciplinary Engineering & Sciences (SINES) at the National University of Sciences and Technology (NUST), Islamabad. With a rich and diverse background in bioinformatics, computational chemistry, systems biology, and automation, Dr. Paracha exemplifies the modern interdisciplinary approach to scientific research and education. His academic journey, which includes a PhD in Applied Biosciences from NUST, has been marked by a commitment to integrating computational sciences with biological research, aiming to bridge the gap between theoretical models and practical applications.
Dr. Paracha’s work is at the cutting edge of several disciplines, focusing on complex areas such as the pathophysiology of sepsis, drug and vaccine design, and the automation of computational processes. His research has led to the development of innovative tools and automated systems that have significantly advanced the fields of computational biology and bioinformatics. As a leading figure in these interdisciplinary domains, he has not only contributed to the scientific community through his prolific publication record in high-impact journals but has also been instrumental in mentoring the next generation of scientists, supervising numerous master’s and doctoral students.
His achievements in the integration of computational sciences with automation in biological research have earned him national recognition, including the prestigious Best Research Paper Award by the Higher Education Commission of Pakistan. Dr. Paracha’s work embodies the future of scientific research, where interdisciplinary collaboration and the use of advanced computational tools are essential for solving complex global challenges.