Techniques in Biotechnology
For industrial professionals
Starting From:
1st Feb to 31 March
Course Description:
Techniques for DNA isolation and manipulation plays an important role in modern day biotechnology.These techniques of biotechnology with different combination can be useful in curing a particular disease, finding biomarkers of the disease and also involved in forensics. In this course all the important and modern biotechnology techniques are described with particular focus on its application and feasibility.
Key Outcomes:
• Cloning and Expression
• Blotting and Hybridization
• DNA Markers
• Specific topics for techniques discussions
• Latest developments in Biotechnology techniques
Trainer Introduction:
Mr. Zeeshan Ahmed is currently working as Junior Scientist of Biotechnology at Green Biotec UG Bremen, Germany since May 2022. Previously, he was a Research Officer/Teaching Assistant at Government College University, Lahore Pakistan and also worked as a researcher at Food and Biotechnology Research center at Pakistan Council of Scientific and industrial research. He has 11 international publications with 51+ impact factor and have expertise in biotechnology techniques like gene cloning, fermentation technology, Immunology, Research methodology, Bioinformatics, Molecular diagnostics, Antibodies development, Antivirals. Virology, Microbial genetics, Recombinant DNA technology, Biosafety and Bioethics, Bioremediation, Immunoassays, Tissue culture, Media optiization, cultivated meat production, Development of protein based downstream and cost estimation of upstream processing, Cell engineering (e.g. genetic engineering of bacteria, yeat and eukaryotic cells) for recombinant proteins production, microbiology, protein purification, and Cloning of genes. Mr. Zeeshan has full command on overlooking all biotechnology labs primarily working of recombinant product production and immunoassays. However, most of his lab and research experience is in bioprocess development, virology, and molecular biology techniques. He has experience with following techniques/lab testing: Isolation and screening or microbes, acid-fast staining, flow cytometry, western blotting, southern blotting, nothern blotting, Agarose gel electrophoresis, SDS-PAGE, Dialysis, Chromatography techniques like HPLC, Ion-exchange etc, Immunoassays like ELISA, RIA etc, Transformation of cells, competent cell formation, Protein purification, Cell groeth analysis, spectrometry, DNS. These testing techniques are usually performed on bispecific antibodies, biosimilars, food and beverages, ex-vivo gene and cell therapy, monoclonal antibodies (MABs), Polyclonal antibodies (PABs), antibodies, dairy products, RNA/mRNA therapy/ vaccines, tissue engineering and isolation. Mr. Zeeshan has expertise in using Bioinformatics software like Pimer3, PyMOL, ClustalOmega, OligoCalc etc. These software and websites are used for Insilco studies of genes and proteins to find their structure and sequence. Moreover, he has expertise in using automated nuclic acid extraction kits for fast extraction of plasmids and chromosomal DNA, QA/QC testing, and automated gel dock systems.
Course Objectives:
To introduce and familiarize students with different techniques used in the field of biotechnology, its practical applications with pros and cons along with the specific requirement of techniques in
different lab and industrial processes
Learning Outcomes:
After completing this course student should be able to:
Understand role of different biotechnology techniques, their practical applications, principle and core steps to perform these techniques in lab and industry.
Course Descriptions:
Week 1-2
Cloning and expression: Introduction; Types of vectors; Recent developments in vectors and
expression system; Chromosomal integration; Phage display libraries; PCR (Standard protocol,
Types and Uses); Protein fractionation: Gel Electrophoresis (Agarose, SDS-PAGE, Pulse field),
HPLC, Pulse field).
Week 3-4
Blotting, hybridization: Identification of macromolecules by Southern, Northern, Western
blotting and Colony hybridization; ELISA; Chemical synthesis of DNA; Gene probes; Sequencing
of DNA; Sequencing of proteins; Protein targeting; Transport and translocation; Protein
Purification.
DNA Markers: DNA markers such as RFLP, RAPD, AFLP and VNTR
Week 5-8
Specific topics: Transgenic mouse; Monoclonal antibodies; Glycoprotein analysis; RNA
interference; DNA Chip Technology.
Latest Developments: Recent advances and future trends in techniques related to
biotechnology along with scenario based practical applications of above-mentioned
techniques
Who Should attend?
Biology scientists
Masters and graduate students seeking knowledge about principles, applications and stpes
involved in various biotechnology techniques.
Industrial professionals
Lab managers
Lab Technicians
Lab Scientists and Researchers
PhD Scholars
Masters’ students acquiring hands on experience of Biotechnology techniques.
