Course 4: Recombinant DNA technology in Cancer Biology
For industrial professionals
Professional Training
Starting from: 1st February
Course 4: Recombinant DNA technology in Cancer Biology For industrial professionals
Course Description:
Recombinant DNA technology has also proven important to the production of vaccines and protein therapies such as human insulin, interferon and human growth hormone. It is also used to produce clotting factors for treating haemophilia and in the development of gene therapy. These recombinant products are used with the main objectives of killing the tumor and stimulating immune cells to respond to the cancer cells. The principal recombinant products in anticancer therapy are immunostimulants, vaccines, antibodies, immunotoxins and fusion proteins. This course will give you a complete understanding of RDT products, its role and function in recognizing, treating and fighting multiple types
of cancers.
Key Points:
- Introduction to Recombinant DNA Technology (RDT) and Introduction: Vectors for Bacteriophage and Overexpression of Foreign Gene
- Gene regulation
- Techniques for Screening of Oncogenes
- DNA synthesis and sequencing
- Specific topics discussion for modern day scholars and scientists
Trainer Introduction:
Mr. Zeeshan Ahmed is currently working as Junior Scientist of Biotechnology at Green Biotec UG Bremen, Germany since May 2022. Previously, he was a Research Officer/Teaching Assistant at Government College University, Lahore Pakistan and also worked as a researcher at Food and Biotechnology Research center at Pakistan Council of Scientific and industrial research. He has 11 international publications with 51+ impact factor and have expertise in biotechnology techniques like gene cloning, fermentation technology, Immunology, Research methodology, Bioinformatics, Molecular diagnostics, Antibodies development, Antivirals. Virology, Microbial genetics, Recombinant DNA technology, Biosafety and Bioethics, Bioremediation, Immunoassays, Tissue culture, Media optiization, cultivated meat production, Development of protein based downstream and cost estimation of upstream processing, Cell engineering (e.g. genetic engineering of bacteria, yeat and eukaryotic cells) for recombinant proteins production, microbiology, protein purification, and Cloning of genes. Mr. Zeeshan has full command on overlooking all biotechnology labs primarily working of recombinant product production and immunoassays. However, most of his lab and research experience is in bioprocess development, virology, and molecular biology techniques. He has experience with following techniques/lab testing: Isolation and screening or microbes, acid-fast staining, flow cytometry, western blotting, southern blotting, nothern blotting, Agarose gel electrophoresis, SDS-PAGE, Dialysis, Chromatography techniques like HPLC, Ion-exchange etc, Immunoassays like ELISA, RIA etc, Transformation of cells, competent cell formation, Protein purification, Cell groeth analysis, spectrometry, DNS. These testing techniques are usually performed on bispecific antibodies, biosimilars, food and beverages, ex-vivo gene and cell therapy, monoclonal antibodies (MABs), Polyclonal antibodies (PABs), antibodies, dairy products, RNA/mRNA therapy/ vaccines, tissue engineering and isolation. Mr. Zeeshan has expertise in using Bioinformatics software like Pimer3, PyMOL, ClustalOmega, OligoCalc etc. These software and websites are used for Insilco studies of genes and proteins to find their structure and sequence. Moreover, he has expertise in using automated nuclic acid extraction kits for fast extraction of plasmids and chromosomal DNA, QA/QC testing, and automated gel dock systems.
Course Objectives:
To acquaint students with basic knowledge of vectors, cloning principles, PCR, cDNA and analytical techniques with their applications in Cancer biology
Learning Outcomes:
After completing this course student should be able to:
Understand Recombinant DNA technology and its scope in Cancer, Acquire knowledge about application of RDT
in biological systems and in cancer biology and future aspects of Cancer treatment and role of RDT in curing cancer.
This course will give understanding of:
Primer designing for gene amplification
Application of RDT in cancer cell biology
RDT product formation
Role of recombinant products in treating multiple myeloma
Gene finding and its analysis
Complete procedure of RDT formation and its role in different types of myeloma
Course Description:
Week 1
Introduction: Vectors for Bacteriophage and Overexpression of Foreign Gene: Bacteriophage λ vectors; Overexpression
of foreign genes in prokaryotes and eukaryotes.
Week 2
Gene Regulation: Regulation of gene expression; Importance of regulatory regions in normal and cancer regions
Week 3
Techniques for Screening of oncogenes: Screening by hybridization; Immunological assay and protein activity in
cancer cells and RDT therapeutics
Week 4-5
Oncogenes and RNAi: Introduction to cancer genomics; Oncogenes; Regulation of oncogenes; RNA interference.
Week 6
DNA Synthesis and Sequencing: Chemical synthesis of DNA; Pyrosequencing; Latest trends in DNA sequencing.
Week 7:
Specific Topics: Fusion proteins; Inclusion bodies; Antibody engineering; Pichia pastoris as an expression system; DNA
shuffling; Nucleic acids as therapeutic agents; Microarrays.
Week 8:
Recent Trends: Recent Development in the field of RDT with respect to Oncogenes and their regulation with future perspectives
Who Should attend?
Biology scientists
Masters and graduate students seeking knowledge about modern day cancer therapeutics and causes of different
types of cancers
Industrial professionals
Lab managers
Lab Technicians
Lab Scientists and Researchers
PhD Scholars
Masters’ students acquiring hands on experience of Recombinant DNA technology.
