Introduction to Practical microbiology
AIMS AND OBJECTIVES:
- The course is designed to enable the students to work with microorganisms.
- The basic techniques of sterilization, culturing, isolation and determining different characteristics of the microorganisms are included.
Course Detail
- Microorganisms and their respective place in the living world.
- Historical development of Microbiology and its scope.
- Microscopy: An outline of the principles and applications of light and electron microscope.
- Morphology, arrangement and detailed anatomy of bacterial cell.
- Bacterial taxonomy and nomenclature, basis of classification of bacteria.
- Growth, nutrition (physical and nutritional requirement and nutritional types; sources of energy, C, N, H, O, S, P, H2O, trace elements, growth factors) and reproduction.
- General methods of studying microorganisms: cultivation, isolation, Purification and characterization.
- Control of microorganisms by physical and chemical methods.
- Chemotherapeutic agents and antibiotics. Modes of action of antibiotics on microorganisms.
- Basic properties of fungi, protozoa and algae.
- A brief introduction to structure and propagation of viruses and bacteriophages.
Microbiology of water and wastewaters. Water as a source of infection and methods of water purification. Methods of sewage treatment and disposal.
- Introduction to food and dairy microbiology. Methods of food preservation.
- Differentiation between food intoxication and food-infection. Microbiology of soil with particular reference to nitrogen cycle.
- Microbiology of air.
Practical:
- Laboratory safety: Containment and decontamination.
- Principles of Staining Procedures: Simple staining, Gram’s staining, Acid- fast staining, cell-wall staining, flagellar staining, capsule staining, spore staining and spirochetes staining.
- Study of cell motility by hanging drop preparation.
- Preparation and sterilization of bacteriological media and glassware.
- Inoculation techniques.
- Study Of colony characteristics of microorganisms.
- Enumeration of bacteria from milk, water, food and soil by standard plate count technique (SPC) and/or most probable number technique (MPN).
- Microbiological analysis of air. Microscopic study of fungi isolated from air.
Recommended Books:
- Kathleen P. T., and Arthur, T. 2001. Foundations in Microbiology: Basic
- Principles McGraw-Hill Companies/
- Tortora, G. J., Funke, B. R., Case, C. L. 2000. Microbiology: An
- Introduction, Study Guide. Benjamin-Cummings Publishing Company.
- Tortora, G. J., Funke , B. R. and Case, C. L. 2004. Microbiology: an
- 8th Introduction Edition, Pearson Education, USA.
- Tortora,G. J., Christine, L. Case, C. L., Funke, B. R., Funke, B., Case, C., 2006. Microbiology: An Introduction, Publisher: Pearson Education.
- Alcamo, I. E., 2001. Fundamentals of Microbiology published by Jones and Bartlett Publishers, USA. 6th
- Black, J. G., 2005. Microbiology: principles and explorations, by Edition, J. Wiley & Sons, USA.
- Cappuccino, J. G. and Sherman, N. 2004, Microbiology: a laboratory manual. Pearson Education, USA.
- Pollack, R. A. Findlay, L., Mondschein, W. Modesto R. R., 2004.
- 2nd Laboratory Exercises in Microbiology by Edition, J. Wiley and Sons, USA.
- Baker , S., Khan , N., Nicklin, J. and Killington, R., 2006. Instant Notes in 3rd Microbiology, Ed edition, Taylor and Francis.
- Madigan, M. T. and Martinko, J., 2005. Brock Biology of Microorganisms 11 International Ed edition Prentice Hall.
- Talaro, K. P., 2006. Foundations in Microbiology: Basic Principles. Mcgraw Hill. Publisher.
