HPLC TROUBLE SHOOTING
ADVANCED LEVEL COURSE
Course Description•: High-performance liquid chromatography or commonly known as HPLC, is an analytical technique used to separate, identify or quantify each component in a mixture.
• The mixture is separated using the basic principle of column chromatography and then identified and quantified by spectroscopy.
• In the 1960s, the column chromatography LC with its low pressure suitable glass columns was further developed to the HPLC with its high-pressure adapted metal columns.
• HPLC is thus basically a highly improved form of column liquid chromatography.
• Despite advancements in HPLC methods and technology, there are still issues that can rear their ugly head when using an HPLC system.
• In this course we will study common problems of HPLC and how to deal with them
Key Points:
Introduction to HPLC
Trouble shooting
Methods to develop HPLC
Trainee Introduction
• I am Tasneem Rashid, a Pharmacist. I had done my graduation of Doctorate of Pharmacy from Hamdard University in 2022.
• Right now I amWorking in Medi sure Pharmaceutical Laboratory as Regulatory Affairs Officer.
• I had some practical experience during my university life as an internee by doing internships in Industrial sector as well as Clinical sector.
• On Industrial side I have an experience as a trainee in Pakistan Council of Scientific And Industrial Research Laboratory Complex Karachi (PCSIR). I had also done an online course of pharmacovigilance.
• On clinical side I have an experience as a trainee pharmacist in Naimat Beghum hospital Karachi, along with onsite experience I had done few online courses in clinical discipline.
• I also have an experience as a trainee pharmacist in community pharmacy.
• During my graduation I have participated in different seminars arranged by Agha khan hospital in collaboration with five IQ.
• I have also participated in under graduate research work.
• I have acquainted myself with a range of skills that would allow me to blend with your company. My internships had
provide me some experience on different pharmacist domain works. I am confident that my knowledge ,positive attitude even under pressure, and precise attention to detail would make me an asset to your company. My organizational, presentation and multitasking skills are all advanced. I am sure that my all these experiences will help me start contributing quickly at your company.
• I can be reached anytime via my email [email protected].
• Lindin Profile: https://www.linkedin.com/in/tasneem-rashid-4204921b9
Course objective
This course expands the participants’ knowledge of HPLC and helps to gain more secureness in handling of HPLC systems. It is intended that afterwards problems can be identified and solved autonomously, entailing more success in method configuration and HPLC analyses.
Learning outcomes
Move beyond standard operating procedures and gain a solid
working knowledge of HPLC principles and methods Build expertise that will help you choose the right column, conditions, detector and separation mechanism to separate difficult mixtures. Improve your on the job performance and skill set Learn basic of HPLC problem and way to dealt with problems by easily identifying them.
Who should attend
Best for:
• R AND D scientist
• QC researcher
• QC officer
• Pharmacist
• Chemist
• Students enroll for mphill in pharmaceutical chemistry, Masters in Chemistry, Masters in Organic Chemistry
Course content
week1
Introduction to HPLC
Introduction operating procedures
week2
Parameters
Week3-4
Types/modes of HPLC
Week5-6
Instrumentation
Week 7
Working principle
Week 8-9
Detection
Week 10
Application
Week 11-14
Trouble shooting
What is mean by trouble shooting Understand/identify problems in
HPLC
Implement measures to prevent
problem occurring
Identify causes
Resolve problem
Week 15-18
Methods to develop HPLC
Introduction
Steps to develop method
Methods developing consideration
Methods developing Resource
Automatic method development
Hardware and software
