Green Biotec UG Bremen Germany
High Performance Liquid Chromatography (HPLC)
| High-Performance Liquid Chromatography (HPLC) | |
|---|---|
| Course Description | High-Performance Liquid Chromatography (HPLC) is an essential analytical tool in various industries, including pharmaceuticals, food science, and environmental analysis. This course provides an in-depth understanding of HPLC principles, instrumentation, and applications. It focuses on method development, optimization, troubleshooting, and practical use cases to ensure participants gain the expertise required for accurate and reliable analysis. |
| Recommended Books | 1. Introduction to Modern Liquid Chromatography by Lloyd R. Snyder, Joseph J. Kirkland, John W. Dolan 2. Practical HPLC Method Development by Lloyd R. Snyder, Joseph J. Kirkland 3. Handbook of HPLC by Elena Katz |
| Course Learning Outcomes | After completing this course, participants will: 1. Understand the theoretical principles and practical applications of HPLC. 2. Gain proficiency in HPLC instrumentation, including maintenance and troubleshooting. 3. Develop and optimize HPLC methods for various analytes. 4. Analyze and interpret chromatographic data with accuracy and confidence. |
| Assessment System | Quizzes: 10-15% Assignments: 5-10% Midterms: 30-40% End Semester Exam: 40-50% |
| Lecture Plan | ||
|---|---|---|
| S.No. | Description | Quizzes/Assignment |
| 1 | Introduction to High-Performance Liquid Chromatography (HPLC) | Quiz 1 |
| 2 | Theoretical Principles of Chromatography | |
| 3 | Components of HPLC: Pumps, Columns, and Detectors | Assignment 1 |
| 4 | Mobile Phase Selection and Preparation | |
| 5 | Method Development in HPLC | |
| 6 | Validation of HPLC Methods | Quiz 2 |
| 7 | Quantitative Analysis Using HPLC | |
| 8 | Troubleshooting Common HPLC Problems | Assignment 2 |
| 9 | Optimization of Chromatographic Conditions | |
| 10 | Advanced HPLC Techniques: Gradient and Isocratic Methods | Quiz 3 |
| 11 | Specialized Applications of HPLC in Pharmaceutical Analysis | |
| 12 | HPLC in Environmental and Food Science | Assignment 3 |
| 13 | Data Analysis and Interpretation of Chromatograms | |
| 14 | HPLC Maintenance and Calibration | |
| 15 | Future Trends in HPLC and Emerging Technologies | |
