Fundamentals of Genetics

Knowledge dissemination & Knowledge Deepening

·       To understand the basic techniques of sterilization, culturing and isolation.

·       Determining different characteristics of the microorganisms.

·       Understand the scope of Practical Microbiology

·       Use the principles of basic techniques use in Microbiology

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·       Microorganisms and their respective place in the living world.

·       Differentiation between pro- and eukaryotic cells.

·       Historical development of microbiology and its scope.

·       Microscopy: An outline of the principles and applications of light and electron microscope.

·       Morphology, arrangement and detailed anatomy of bacterial cell.

·       Bacterial staining and different type of staining, Gram positive, Gram negative, Acid fast staining.

·       Bacterial taxonomy and nomenclature, basis of classification of bacteria.

·       Growth, nutrition (physical and nutritional requirement and nutritional types; sources of energy, C, N, H, O, S, P, H O, trace elements, growth factors) and reproduction.

·       General methods of studying microorganisms: cultivation, isolation, purification and characterization.

·       Control of microorganisms by physical and chemical methods.

·       Chemotherapeutic agents and antibiotics. Modes of action of antibiotics on microorganisms.

·       Basic properties of fungi, protozoa and algae. A brief introduction to structure and propagation of viruses and bacteriophages.

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2.  An introduction to microscopy.

3. Principles of Staining Procedures: Simple staining, Gram’s staining, Acid-fast staining, cell wall staining, flagellar staining, capsule staining, spore staining and spirochaete staining. Study of cell motility by hanging drop preparation.

4. Preparation and sterilization of bacteriological media and glassware.

5. Inoculation techniques. Study of colony characteristics of microorganisms.

6. Standard plate count technique (SPC).

7. Microbiological analysis of air.

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2.   Black, J. G. 2005. Microbiology: Principles  and  Explorations, 6th edition, John Wiley and Sons, N.Y.

3.   Talaro, K. P. 2008. Foundations in Microbiology: Basic Principles, McGraw-Hill Companies, N.Y.

4.   Tortora, G. J., Funke, B. R. and Case, C. L. 2012. Microbiology: An Introduction, Benjamin-Cummings Publishing Company, U.S.A.

 Written Assignments.

 Class activities and discussion.Practical hands on practice or video demonstration

Mid Term (40%)

 Written (Long Questions, Short Questions, MCQs)

 Project/case study/Presentation

 Assignments

 Tests/Quiz. / Viva

Final Term (60%)

 Written (Long Questions, Short Questions, MCQs).

Total working hours=180                Credit points= 3(2+1)